Pharmaceutical Analysis



Department of Pharmaceutical Analysis

Grace College of Pharmacy, Palakkad established Department of Pharmaceutical Analysis for the first time in Kerala under self financing in 2010, realizing the importance of the subject to the Pharmaceutical and Allied Industry. The permission for the same was granted by AICTE and PCI in 2010 which was subsequently affiliated by University of Calicut.


Analysis of drugs and pharmaceutical forms the backbone of research and development in pharmaceutical industry. The department focuses on training the graduate and post graduate students on how to use the moderns and newer instrumental analytical techniques such as HPLC, FT-IR, UV-Visible Spectroscopy, Flourimetery, Flame Photometry, Electrophoresis etc. Special emphasis is laid on the implementation on good laboratory practices and analytical skills. The analysis department is well equipped and has all the features to get recognized as center for developing new analytical methods and thrie validation, characterization and quantitative estimation of possible impurities in various dosage forms.

Research focus:

Analysis of pharmaceuticals are faced with every increasing demands fro more sensitive and specific methods to detect the increasing number of clinical agents. Modern analytical Chemistry, despite its apparent multidisciplinary character, is a unified and independent scientific discipline which participates with other sciences in optimization and development of many processes in the modern technology. Today, modern pharmaceutical analysis entails good understanding of the physiochemical behavior of solids to ensure a better selection of formulation. The analytical methodology, the experiments are performed to standardize and then obtain a procedure capable of qualifying a given substance. While developing analytical methodologies, we validate the method by determining important paradigms viz., potency, stability, purity and others. Thus we define the control system and the suitable and appropriate control parameters and techniques are selected for method development. We have developed new analytical assay techniques for drugs like Darunavir, Duloxetine hudrochloride, Telmisartan, Indapamide, Amlodipine and others.

Bioanalysis plays a special vital role in pharmacokinetics, bioavailability and bioequivalence studies. With the use of newer analytical techniques, we have been actively involved in the study of new drugs molecules and their estimation in human plasma for various pharmaceutical industries. The pharmaceutical Analysis laboratory is closely associated with the bioanalysis of blood, plasma, serum for therapeutic drug monitoring and determining drug levels in human beings. Such analysis is carried out by standardization, optimization and validation, keeping the entire quality control requirement.

Analytical service to Industry:

Postgraduate students research activities of Department of Pharmaceutical Analysis are focused on:

  • Herbal standardization by identifying biomarkers.
  • Phytochemical isolation and characterization from potential unexplored plant flora.
  • Analytical method development.
  • Impurity profile studies of some known drugs.


  • BL-198 bio spectrophotometer – Elico Ltd.
  • UV – 1800 Shimabzu UV spectrophotometer – Toshvin Analytical Pvt Ltd.
  • IR Affinity – 1, Fourier transform spectrometer - Toshvin Analytical Pvt Ltd.
  • HPLC – Shimabzu – Spinco biotech Pvt. Ltd.
  • pH meter – Elico Ltd.
  • KF Tirator – Systronics 89-92 industrial area.
  • Calorimeter – Systronics.
  • Conductivity meter – Elico Ltd.
  • Photo fluori meter - Systronics.
  • Ultrasonic cleaner –Elico Ltd.
  • Flame photometer –Systronics.
  • Hot air oven – Technico.
  • Turbidimetre – Mode 331 – E.


  • Cicy Eapen1, Prasanth V G*2, and Amita Rai3, Development Of UV Spectrometric method Of Glibenclamide (Glyburide) in bulk and pharmaceutical formulations, International Journal of ChemTech Research , Jan-Mar 2012, Vol.4, No.1, pp. 356-360
  • V G Prasanth*, ,Susamma Cicy Eapen, Sheeja Velayudhan Kutty, T S Jyothi, Development and validation of UV-Spectrophotometric method for determination of Quetiapine fumarate in pure and pharmaceutical formulation, ijpt | dec-2011 | vol. 3 | issue no.4 | 3535-3542
  • Prasanth vg*1, Susamma Cicy Eapen, Sheeja Velayudhan Kutty and T S Jyothi, Development and validation of Quetiapine fumarate in pure and pharmaceutical formulation by UV-Spectrophotometric method, Pelagia Research Library, Der Pharmacia Sinica, 2011, 2 (6):52-58M
  • Mr.Prasanth V G *, Mrs.Sheeja Velayudhan kutty, Mrs. Susamma Cicy Eapen, Ms.Akhila Baby, , Development of validated uv-visible Spectrophotometric method for the estimation Of fenofibrate in pure and pharmaceutical Formulation using MBTH reagent, International journal of pharmaceutical research and bio-science is provisionally accepted for Volume 3, Issue 1 ,January-March 2012.


  • K.R.Sini, Rai Amita, Y.Haribabu, K.S. Saneesh, Determining the antioxidant activity of certain medicinal plants of Palakkad, India using DPPH assay.
  • K.R.Sini, Anusha Ajayakumar, Jithamol.k.p, Ragisha Francis, Ethno botanical Study of Medicinal Plants Used by the Irula and Muruga Tribes of Attapady Region in India.
  • Ragisha Francis, Divya D,Jyothi T.S, Prasanth V.G, A validated HPLC method for the simultaneous estimation of Rabeprazole sodium and Itopride HCl in their capsule dosage form.
  • K.R.Sini ,N.Chandrasekhar, Jaya prakash Varma,Antipyretic effects of leaves of Secamone emetica (RETZ) R.BR.EX SCHULT
  • Paper presented at St.James college of Pharmacy Chalakkudy 2010
  • Jithamol k.p, Sangeetha P.T, Pharmacognostical Investigation and Anti-Microbial activity of Aqueous and Hydroalcoholic extract of leaves of Asystasia violacea”
  • Sini K R and Jyothi T S, Study of the Anti-diarrhoeal activities of selected medicinal plants used by the Irular Tribes of Palakkad,South India.
  • Sangeetha p.t, Arshana. P.G, In-vitro anti-oxidant activity of various leaf extracts of Asystasia violacea.